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EGFR Del19/T790M/C797S overexpressed Stable PC9 Cell Line

Catalog Number CBCL-256
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

PC9 cell line bearing exogenous EGFR Del19/T790M/C797S mutations.

Application

Drug screening and biological assays

Background

EGFR, the Epidermal growth factor receptor, is a cell-surface receptor tyrosine kinase. EGFR overexpression or overactivity have associated witha number of cancers, including the lung cancer and colon cancer. The identification of EGFR as a driver gene has led to the development of anticancer therapeutics agents.

Product Information

Species Human
Host Cell Line PC9
Cell Type Human non-small cell lung cancer cell line
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Kinases
Recommended Medium RPMI1640 + 10% FBS + 0.5 μg/ml Puromycin
Selection Marker Puromycin
Morphology Epithelial-like
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:3-1:6.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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