Contact Us

USA

Tel:
Fax:
Email:
Europe
Tel:
Email:

;

NIH3T3 human KRAS Stable Ba/F3 Cell Line

Catalog Number CBCL-257
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable NIH3T3 clone expressing exogenous human K-ras sequence.

Application

Drug screening and biological assays

Background

The KRAS gene provides instructions for making a protein called K-Ras that is part of a signaling pathway known as the RAS/MAPK pathway. The overactivation due to mutation can lead to continuous cell proliferation, and eventually develop into cancer.

Product Information

Species Mouse
Host Cell Line Ba/F3 cell line
Cell Type Pro-B cells
Stability Stable in culture over 10 passages
Growth Properties Suspension
Pharmaceutical Target Kinases
Recommended Medium RPMI 1640 + 10% FBS
Selection Marker Puromycin
Morphology Mostly round (some polymorph) cells
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:10.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 20 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
Copyright © 2024 Creative Bioarray. All rights reserved.