Introduction
Recombinant 1321N1 cells have been developed to stably overexpress human pyrimidinergic receptor P2Y11 (P2Y11) on their surface. These cells contain high levels of the G protein Gαq, which is crucial for coupling with the receptor in downstream signaling pathways.
Background
P2Y11 is a G-protein coupled receptor predominantly expressed on the surface of various immune cells, including dendritic cells, macrophages, and natural killer (NK) cells, as well as in certain other tissues. It functions as a critical receptor for extracellular nucleotides, particularly adenosine triphosphate (ATP) and its derivatives, playing a pivotal role in immune regulation and inflammation. Upon activation by its ligands, P2Y11 triggers signaling pathways that involve both phospholipase C and adenylyl cyclase, leading to increased intracellular calcium levels and cyclic AMP production, which in turn modulate cytokine secretion, cell survival, and immune responses.
