Non-CYP Mediated Metabolism

In recent years, significant progress has been made in reducing metabolic instability due to cytochrome P450-mediated oxidation. High-throughput metabolic stability screening has enabled the advancement of compounds with little or no oxidative metabolism. In addition, high lipophilicity and low aqueous solubility of presently pursued chemotypes reduce the likelihood of renal excretion. Therefore, these low microsomal turnover compounds are generally substrates for non-CYP-mediated metabolism. Here, we focus on the utility of appropriate in vitro studies to characterize non-CYP-mediated metabolism and to understand the enzymes involved in pharmacokinetic studies in appropriately characterized surrogate species. We have developed a suite of validated in vitro assays using recombinant enzyme systems or cellular fractions with specific chemical inhibitors for phenotypic analysis of non-CYP metabolic pathways and assessment of DDI potential.

Available reaction phenotyping services include:

• Flavin monoxygenases (FMOs)
• Monoamine oxidases (MAOs)
• Esterases, including carboxylesterases (CEs), cholinesterases (acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)) and paraoxonase (PON)
• Aldehyde oxidase (AO)
• Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH)
• Aldo-keto reductases (AKR)
• UDP-glucuronyl transferases (UGT)
• Sulpho transferases (SULT)
• N-Acetyl transferases (NAT)
• Glutathione S-transferases (GST)
• Methyl transferases
• Liver microsomes
• S9
• Cytosol
• Hepatocytes
• Extrahepatic tissues

Creative Bioarray's non-CYP mediated metabolism services enable a greater understanding of which non-CYP enzymes might be involved in the metabolism of your compounds, or whether your compounds are inhibitors of non-CYP enzymes. These data can be used to determine potential drug-drug interactions. In addition, other enzymes can also be investigated upon request.

Related Services:

• CYP Induction Assay
• CYP Inhibition Assay
• In Vitro Permeability Assay
• Lipophilicity & Solubility
• Cell Line Panel
• Red Blood Cell Partitioning
• Plasma Protein Binding Assay
• Plasma Stability Assay
• In Vitro Metabolic Stability