Introduction
Recombinant CHO-K1 cells have been engineered to stably overexpress human adenosine A2A receptor (ADORA2A) on their surface. These cells contain high levels of the G protein Gαs, which is essential for coupling with the receptor in downstream signaling pathways.
Background
The ADORA2A gene encodes the adenosine A2A receptor, a G protein-coupled receptor. It is highly expressed in the brain, especially in the striatum, and also in immune cells, blood vessels, and other tissues. In the brain, activation of A2A receptors modulates neurotransmitter release, such as dopamine and glutamate, and is involved in motor control, reward - related behaviors, and sleep regulation. In immune cells, A2A receptors have anti - inflammatory effects by inhibiting the production of pro - inflammatory cytokines. A2A receptor antagonists are being developed as potential drugs for Parkinson's disease and other neurological disorders.
