Introduction
Recombinant CHO-K1 cells have been engineered to stably overexpress human adrenoceptor alpha 2A (ADRA2A) on their surface. These cells contain high levels of the G protein Gαi, which is essential for coupling with the receptor in downstream signaling pathways.
Background
The ADRA2A gene encodes the alpha - 2A adrenergic receptor, a G protein-coupled receptor. It is widely distributed in the central and peripheral nervous systems, as well as in adipose tissue. In the nervous system, activation of ADRA2A receptors inhibits neurotransmitter release, such as norepinephrine, leading to a decrease in sympathetic nervous system activity. This results in effects such as reduced heart rate, lower blood pressure, and sedation. In adipose tissue, it inhibits lipolysis. ADRA2A receptor agonists are used as antihypertensive and sedative drugs, while antagonists can be used to increase sympathetic tone in certain conditions.
