CHO-K1-CCR5/Gα15-Stable Cell Line
| Catalog Number |
CBCL-0084 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
A CHO-K1 cell line has been engineered to stably overexpress human C-C motif chemokine receptor 5 (CCR5) on its surface. This cell line contains high levels of the G protein Gαi, which is essential for coupling with the receptor in downstream signaling pathways.
Application
Drug screening and biological assays
Background
The CCR5 gene encodes a chemokine receptor that is a co - receptor for the entry of human immunodeficiency virus (HIV) into host cells. It is expressed on the surface of T lymphocytes, macrophages, and other immune cells. CCR5 binds to chemokines such as CCL3, CCL4, and CCL5. In addition to its role in HIV infection, CCR5 is involved in the regulation of immune cell trafficking during inflammation and immune responses. Genetic variations in the CCR5 gene can affect an individual's susceptibility to HIV infection and the progression of AIDS.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-CCR5/Gα15-Stable Cell Line |
| Protein Family |
Chemokine receptor |
| Gene |
CCR5 |
| NCBI Gene ID |
1234 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 3 µg/ml puromycin, 100 µg/ml Hygromycin B |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
