CHO-K1-CXCR4/Gα15-Stable Cell Line
| Catalog Number |
CBCL-0087 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
A CHO-K1 cell line has been developed to stably overexpress human C-X-C motif chemokine receptor 4 (CXCR4) on its surface. This cell line contains high levels of the G protein Gαi, which is crucial for coupling with the receptor in downstream signaling pathways.
Application
Drug screening and biological assays
Background
The CXCR4 gene, also known as CD184, encodes a chemokine receptor that is widely expressed on various cell types, including hematopoietic stem cells, lymphocytes, and endothelial cells. It binds to the chemokine CXCL12 (stromal cell - derived factor 1). Activation of CXCR4 by CXCL12 regulates cell migration, homing of hematopoietic stem cells to the bone marrow, and the trafficking of immune cells during development and immune responses. CXCR4 is also involved in processes such as angiogenesis and tumor metastasis.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-CXCR4/Gα15-Stable Cell Line |
| Protein Family |
Chemokine receptor |
| Gene |
CXCR4/CD184 |
| NCBI Gene ID |
7852 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin, 100 μg/ml Hygromycin B |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
