CHO-K1-Gα15/AMY1-Stable Cell Line
| Catalog Number |
CBCL-0076 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
A CHO-K1 cell line has been genetically engineered to stably overexpress human receptor activity modifying protein 1 (RAMP1) and calcitonin receptor (CTR) on its surface. Amylin receptors (AMYR), which are unique protein heterodimers consisting of a CTR paired with one of three receptor activity-modifying proteins (RAMP1, RAMP2, RAMP3), form three distinct subtypes (AMY1, AMY2, AMY3).
Application
Drug screening and biological assays
Background
The AMY1 gene encodes salivary amylase, an enzyme that initiates the digestion of starches in the mouth. It breaks down complex carbohydrates into smaller polysaccharides and oligosaccharides. The expression level of AMY1 can vary among individuals and populations, and it has been associated with differences in dietary starch intake and adaptation to different diets.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-Gα15/AMY1-Stable Cell Line |
| Protein Family |
Calcitonin receptor |
| Gene |
AMY1 |
| NCBI Gene ID |
276 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
