HEK293-Human GCGR (Luc)-Reporter Cell
| Catalog Number |
CBCL-0028 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
A recombinant HEK293 cell line expressing the human glucagon receptor (GCGR) fused with a luciferase reporter gene. This cell line is used to study the activation of the GCGR receptor and its downstream signaling pathways through luciferase activity.
Application
Screen for agonists that can bind and activate GCGR.
Background
The GCGR gene encodes the glucagon receptor, a G protein-coupled receptor. It is mainly expressed in the liver and pancreas. Glucagon binds to its receptor and activates a signaling cascade that leads to the breakdown of glycogen into glucose (glycogenolysis) and the production of glucose from non-carbohydrate sources (gluconeogenesis), thereby increasing blood glucose levels. Dysregulation of glucagon signaling is associated with metabolic disorders, such as diabetes mellitus.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
HEK293-Human GCGR (Luc)-Reporter Cell |
| Protein Family |
Glucagon receptor family |
| Gene |
GCGR |
| NCBI Gene ID |
2642 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
