HEK293-Human GIPR (Luc)-Reporter Cell
| Catalog Number |
CBCL-0032 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
A recombinant HEK293 cell line expressing the human glucose-dependent insulinotropic polypeptide receptor (GIPR) fused with a luciferase reporter gene. This cell line is used to study the activation of the GIPR receptor and its downstream signaling pathways through luciferase activity.
Application
Screen for agonists that can bind and activate GIPR.
Background
The GIPR gene encodes the glucose-dependent insulinotropic polypeptide (GIP) receptor, a G protein-coupled receptor. It is expressed in pancreatic beta cells. GIP is secreted by the small intestine in response to food intake and binds to its receptor on beta cells, stimulating insulin secretion in a glucose-dependent manner. GIP also has other metabolic effects, such as promoting fat storage and inhibiting gastric emptying. Dysfunction of the GIP signaling pathway is associated with obesity and type 2 diabetes.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
HEK293-Human GIPR (Luc)-Reporter Cell |
| Protein Family |
Class B G-protein-coupled receptor |
| Gene |
GIPR |
| NCBI Gene ID |
2696 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
