HEK293-Human TrkA (Luc)-Reporter Cell
| Catalog Number |
CBCL-0189 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The Human TrkA (Luc) HEK293 Reporter Cell has been designed to express the SRE signaling response element and the full-length human TrkA (Uniprot: P04629-1). When stimulated with human NGF protein, the NGF/TrkA interaction drives SRE-mediated luminescence. Neutralization of the biological effect of human NGF protein by corresponding antibodies results in a decrease in luminescence.
Application
Screen for neutralizing antibodies blocking the stimulation of human NGF protein.
Background
Tropomyosin receptor kinase A (TrkA) is a receptor tyrosine kinase that binds nerve growth factor (NGF), involved in the development and maintenance of the nervous system.
Product Information
| Category |
Receptor Tyrosine Kinase Cell Lines |
| Cell Line Name |
HEK293-Human TrkA (Luc)-Reporter Cell |
| Protein Family |
Neurotrophic tyrosine receptor kinase |
| Gene |
TrkA |
| NCBI Gene ID |
4914 |
| Species |
Human |
| Host Cell Line |
HEK293 |
| Cell Type |
Human embryonic kidney cells |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (40 μg/mL) |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
