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Human CD73-PDL1 Stable CT26 Cell Line

Catalog Number CBCL-310
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable CT26 clone expressing exogenous human CD73 PDL1 gene

Application

Drug screening and biological assays

Background

CD73 is a glycosylphosphatidylinositol (GPI) anchored cell surface protein that is encoded by NTSE gene, which plays a crucial role in switch on adenosinergic signaling. This gene can also be used as a key regulatory molecule in cancer development. PDL1 is a type I transmembrane protein which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Recently, some results further support the combination of anti-CD73 and anti-PD-L1 therapies in treating EGFR-mutated NSCLC, while suggesting that increased T cell activity may play a role in response to therapy.

Product Information

Species Mouse
Host Cell Line CT26 cell line
Cell Type Mouse colon cancer cell line
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium RPMI1640 + 10% FBS + 10 μg/ml Puromycin + 600 μg/ml Hygromycin B
Selection Marker Puromycin & Hygromycin B
Morphology Fibroblast
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:10.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 20 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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