Catalog Number | CBCL-310 |
Price | Online Inquiry |
Unit Quantity | 1x106 cells / 1.0 ml |
Stable CT26 clone expressing exogenous human CD73 PDL1 gene
Drug screening and biological assays
CD73 is a glycosylphosphatidylinositol (GPI) anchored cell surface protein that is encoded by NTSE gene, which plays a crucial role in switch on adenosinergic signaling. This gene can also be used as a key regulatory molecule in cancer development. PDL1 is a type I transmembrane protein which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Recently, some results further support the combination of anti-CD73 and anti-PD-L1 therapies in treating EGFR-mutated NSCLC, while suggesting that increased T cell activity may play a role in response to therapy.
Species | Mouse |
Host Cell Line | CT26 cell line |
Cell Type | Mouse colon cancer cell line |
Stability | Stable in culture over 10 passages |
Growth Properties | Adherent |
Pharmaceutical Target | Immune Molecules |
Recommended Medium | RPMI1640 + 10% FBS + 10 μg/ml Puromycin + 600 μg/ml Hygromycin B |
Selection Marker | Puromycin & Hygromycin B |
Morphology | Fibroblast |
Seeding Density | Thaw entire contents into an appropriate T25 flask. |
Subculture Ratio | Recommended a ratio of 1:10. |
Storage Method | Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
Doubling Time | Approximately 20 hours |
QC | WB |
Biosafety Level | BSL-1 |
Shipping | Shipping in dry ice |
Caution | For Research Use Only |