TAAR1 Stable HEK293-cAMP-biosensor-membrane Cell Line

Catalog Number	CBCL-319
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

HEK293-cAMP-biosensor-22F cell line stable expressing exogenous TAAR1 gene with membrane signal peptide and HA tag

Application

Drug screening and biological assays

Background

Trace-amine associated receptor 1 (TAAR1) is the best-characterized member of the family of TAARs. TAAR1 is widely expressed across the mammalian brain, particularly in limbic and monoaminergic areas, allegedly involved in mood, attention, memory, fear, and addiction. There is evidence to suggest that TAAR1 may be a promising therapeutic target for the treatment of schizophrenia, psychosis in Parkinson's disease, substance use disorders, and the metabolic syndrome and obesity.

Product Information

Species	Human
Host Cell Line	KC-2177-HEK293-cAMP-biosensor-22F
Cell Type	Human embryonic kidney cells
Stability	Stable in culture over 10 passages
Growth Properties	Adherent
Pharmaceutical Target	Immune Molecules
Recommended Medium	DMEM + 10% FBS + 100 μg/ml Hygromycin B + 9 μg/ml Puromycin
Selection Marker	Puromycin & Hygromycin B
Morphology	Epithelial
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time	Approximately 48 hours
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.