1321N1-P2Y12/Gα15-Stable Cell Line

Introduction

Recombinant 1321N1 cells have been created to stably overexpress human purinergic receptor P2Y12 (P2Y12) on their surface. These cells contain high levels of the G protein Gαi, which is essential for coupling with the receptor in downstream signaling pathways.

Application

Drug screening and biological assays

Background

P2Y12 is a G-protein coupled receptor primarily expressed on the surface of platelets. It serves as a crucial receptor for adenosine diphosphate (ADP). Upon binding of ADP, P2Y12 initiates a signaling cascade that leads to platelet activation and aggregation, which is essential for normal hemostasis but also contributes to pathological thrombosis. It is a key target for antiplatelet drugs such as clopidogrel, which are used to prevent cardiovascular events like heart attacks and strokes.

Product Information