1321N1-P2Y12/Gα15-Stable Cell Line
| Catalog Number |
CBCL-0148 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
Recombinant 1321N1 cells have been created to stably overexpress human purinergic receptor P2Y12 (P2Y12) on their surface. These cells contain high levels of the G protein Gαi, which is essential for coupling with the receptor in downstream signaling pathways.
Application
Drug screening and biological assays
Background
P2Y12 is a G-protein coupled receptor primarily expressed on the surface of platelets. It serves as a crucial receptor for adenosine diphosphate (ADP). Upon binding of ADP, P2Y12 initiates a signaling cascade that leads to platelet activation and aggregation, which is essential for normal hemostasis but also contributes to pathological thrombosis. It is a key target for antiplatelet drugs such as clopidogrel, which are used to prevent cardiovascular events like heart attacks and strokes.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
1321N1-P2Y12/Gα15-Stable Cell Line |
| Protein Family |
P7Y receptor |
| Gene |
P2Y12 |
| Species |
Human |
| Host Cell Line |
1321N1 |
| Cell Type |
Human astrocytoma cell line |
| Morphology |
Fibroblast-like |
| Growth Properties |
Adherent |
| Recommended Medium |
DMEM, 10% FBS, 200 μg/ml Zeocin, 50 μg/ml Hygromycin B |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
