A549-EGFR Knockout-Stable Cell Line
| Catalog Number |
CBCL-0647 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
The A549-EGFR Knockout-Stable Cell Line is an A549 lung cancer cell line in which human EGFR (epidermal growth factor receptor) is no longer expressed due to CRISPR/Cas9 genome editing. This cell line was generated by electroporation of A549 cells with ribonucleoprotein complexes of Cas9 and sgRNAs targeting human EGFR.
Application
Drug screening and biological assays
Product Information
| Category |
Tyrosine Kinases Cell Lines |
| Cell Line Name |
A549-EGFR Knockout-Stable Cell Line |
| Protein Family |
Epidermal growth factor receptor family |
| Gene |
EGFR |
| Species |
Human |
| Host Cell Line |
A549 |
| Cell Type |
Human lung carcinoma |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
Cell Freezing Medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
