Catalog Number | CBCL-249 |
Price | Online Inquiry |
Unit Quantity | 1x106 cells / 1.0 ml |
Stable Ba/F3 clone expressing exogenous BCR-ABL gene bearing the E255V point mutation on the ABL gene.
Drug screening and biological assays
BCR-ABL is an abnormal protein mostly found in chronic myelogenous leukemia (CML). BCR-ABL and its mutants can promote and maintain the malignant behavior of the cancer cells. The identification of BCR-ABL as a driver gene has led to the rapid development of anticancer therapeutics agents. Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.
Species | Mouse |
Host Cell Line | Ba/F3 cell line |
Cell Type | Pro-B cells |
Stability | Stable in culture over 10 passages |
Growth Properties | Suspension |
Pharmaceutical Target | Kinases |
Recommended Medium | RPMI 1640 + 10% FBS |
Selection Marker | Puromycin |
Morphology | Mostly round |
Seeding Density | Thaw entire contents into an appropriate T25 flask. |
Subculture Ratio | Recommended a ratio of 1:10. |
Storage Method | Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
Doubling Time | Approximately 20 hours |
QC | WB |
Biosafety Level | BSL-1 |
Shipping | Shipping in dry ice |
Caution | For Research Use Only |