BHK 21-CTR (Luc)-Reporter Cell Development Service
| Catalog Number |
CBCL-0234 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The Human CTR (Luc) BHK 21 Reporter Cell was engineered to express the CREB signaling response element and the full-length human calcitonin receptor (CTR) (Uniprot: P30988-2). This cell line can drive luciferase expression in response to amylin receptor (AMYR) agonists or amylin stimulation. In the absence of agonists or amylin, the CTR receptor is inactive, resulting in low luminescence. In the presence of agonists or amylin, dose-dependent CTR pathway-activated luminescence can be detected.
Application
Screen for AMYR agonists that can bind and activate CTR.
Background
CTR (Calcitonin Receptor) is a G protein-coupled receptor that binds calcitonin and regulates calcium homeostasis.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
BHK 21-CTR (Luc)-Reporter Cell Development Service |
| Protein Family |
Calcitonin receptor family |
| Gene |
CTR |
| Species |
Hamster |
| Host Cell Line |
BHK21 |
| Cell Type |
Baby Hamster Kidney fibroblast |
| Morphology |
Fibroblast-like |
| Growth Properties |
Adherent |
| Selection Marker |
Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
DMEM + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
