BRAF-V600_K601delinsE Stable Ba/F3 Cell Line

Catalog Number	CBCL-59
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

Stable Ba/F3 clone expressing exogenous BRAF with V600_K601del-ins-E mutation.

Application

Drug screening and biological assays

Background

BRAF is a gene found on chromosome seven that encodes a protein also called BRAF. This protein plays a role in cell growth by sending signals inside the cell promoting, among other functions, cell division. The identifcaiton of BRAF as a oncogene has led to the repaid deviopement of several therapeutical anticancer drugs, such as vemurafenib and dabrafenib. Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.

Product Information

Category	Kinase Cell Lines
Cell Line Name	BRAF-V600_K601delinsE Stable Ba/F3 Cell Line
Pharmaceutical Target	Kinases
Species	Mouse
Host Cell Line	Ba/F3
Cell Type	Pro-B cells
Morphology	Mostly round
Growth Properties	Suspension
Selection Marker	Puromycin
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:10.
Doubling Time	Approximately 20 hours
Recommended Medium	RPMI 1640 + 10% FBS
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability	Stable in culture over 10 passages
QC	WB
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.