CD 16a-V158 Stable NK-92MI Cell Line

Catalog Number	CBCL-551
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

Stable NK-92MI clone expressing exogenous human FCGR3A amino acid bearing V158 mutation

Application

Drug screening and biological assays

Background

The Antibody-dependent cellular cytotoxicity (ADCC), is an important mechanism of immune defenses, an effector cell of immune system, especially the natural killer cells, actively kill a target cell whose membrane-surface antigens have been bound by specific antibodies. NK92MI line is an immortal natural killer cell line but absence of Fc receptors CD16a, which bind to the Fc part of an IgG antibody. After exogenous expressing human high affinity FC receptor mutant CD16a-V158, the NK92MI line can kill the target cell in ADCC assay after the specific antibody has bound to the antigen in the surface of a target cell.

Product Information

Species	Human
Host Cell Line	NK-92MI cell line
Cell Type	Human natural killer cells
Stability	Stable in culture over 10 passages
Growth Properties	Suspension
Pharmaceutical Target	Immune Molecules
Recommended Medium	αMEM + 20% FBS + 2 mM L-glutamine + 1.5 g/L sodium bicarbonate + 0.2 mM inositol + 0.1 mM B-ME + 0.02 mM folic acid + 1 μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Mostly round
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:3.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.