CHO-Human CD32b-High Expression-Stable Cell Line
| Catalog Number |
CBCL-0013 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
A CHO cell line has been created to stably express the full - length human CD32b (Uniprot: P31994-1) receptor. This cell line has different levels of CD32b expression (High, Medium, Low), which can be used to test whether an agonist antibody can enhance its agonistic activity in a CD32b - dependent manner.
Application
Useful for cell-based CD32b binding assay
Background
FCGR2B encodes an inhibitory receptor for the Fc region of IgG. It is expressed on B cells, macrophages, and dendritic cells. This receptor contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic tail. When the receptor is engaged by immune complexes, it recruits phosphatases that inhibit the activation signals generated by other immune receptors, thereby dampening the immune response. It plays a crucial role in maintaining immune tolerance and preventing autoimmune diseases.
Product Information
| Category |
Fc Receptor Cell Lines |
| Cell Line Name |
CHO-Human CD32b-High Expression-Stable Cell Line |
| Protein Family |
Fc gamma receptor |
| Gene |
CD32b/FCGR2B |
| NCBI Gene ID |
2213 |
| Species |
Hamster |
| Host Cell Line |
CHO |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Hygromycin B (20 μg/mL) |
| Recommended Medium |
F-12K+ 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
