CHO-K1-5-HT1A/Gα15-Stable Cell Line
| Catalog Number |
CBCL-0048 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
Recombinant CHO-K1 cells have been developed to stably overexpress human 5-hydroxytryptamine receptor 1A (5-HT1A) on their surface. These cells also contain high levels of the G protein Gαi, which is essential for coupling with the receptor in downstream signaling pathways.
Application
Drug screening and biological assays
Background
This gene encodes a G protein-coupled receptor for serotonin (5-hydroxytryptamine, 5-HT). It is involved in regulating mood, sleep, pain perception, and cognitive function. Abnormal function of this receptor is associated with depression, anxiety, and schizophrenia.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-5-HT1A/Gα15-Stable Cell Line |
| Protein Family |
5-Hydroxytryptamine receptor |
| Gene |
5-HT1A/HTR1A |
| NCBI Gene ID |
3350 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin , 100 μg/ml Hygromycin |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
