CHO-K1-5-HT2B-Stable Cell Line
| Catalog Number |
CBCL-0050 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
Recombinant CHO-K1 cells have been created to stably overexpress human 5-hydroxytryptamine receptor 2B (5-HT2B) on their surface. These cells contain high levels of the G protein Gαq, which is essential for coupling with the receptor in downstream signaling pathways.
Application
Drug screening and biological assays
Background
This gene encodes one of several different receptors for serotonin that belong to the G-protein coupled receptor 1 family. Serotonin receptors mediate many of the central and peripheral physiologic functions of serotonin, including regulation of cardiovascular functions and impulsive behavior.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-5-HT2B-Stable Cell Line |
| Protein Family |
5-Hydroxytryptamine receptor |
| Gene |
5-HT2B/HTR2B |
| NCBI Gene ID |
3357 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin , 100 μg/ml Hygromycin |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
