Introduction
Recombinant CHO-K1 cells have been developed to stably overexpress human adenosine A1 receptor (ADORA1) on their surface. These cells contain high levels of the G protein Gαi, which is crucial for coupling with the receptor in downstream signaling pathways.
Background
The ADORA1 gene encodes the adenosine A1 receptor, which is a G protein-coupled receptor. It is widely distributed in the central nervous system, heart, kidneys, and adipose tissue. Activation of A1 receptors has various effects, including inhibition of neurotransmitter release in the brain, reduction of heart rate and contractility, and inhibition of lipolysis in adipose tissue. It plays a role in regulating sleep - wake cycles, pain perception, and cardiovascular function. A1 receptor agonists are being investigated as potential therapeutic agents for conditions such as arrhythmias and neurodegenerative diseases.
