Introduction
Recombinant CHO-K1 cells have been developed to stably overexpress human adenosine A2B receptor (ADORA2B) on their surface. These cells contain high levels of the G protein Gαs, which is essential for coupling with the receptor in downstream signaling pathways.
Background
The ADORA2B gene encodes the adenosine A2B receptor, a G protein-coupled receptor. It is expressed in a variety of tissues, including the lungs, intestines, kidneys, and immune cells. In the lungs, activation of A2B receptors can cause bronchodilation and has anti - inflammatory effects. In the intestines, it is involved in regulating intestinal fluid secretion and motility. In immune cells, A2B receptors modulate the immune response by influencing cytokine production and cell activation. A2B receptor agonists and antagonists are being explored for the treatment of respiratory diseases, inflammatory bowel diseases, and other conditions.
