Introduction
Recombinant CHO-K1 cells have been created to stably overexpress human adrenoceptor alpha 2B (ADRA2B) on their surface. These cells contain high levels of the G protein Gαi, which is crucial for coupling with the receptor in downstream signaling pathways.
Background
The ADRA2B gene encodes the alpha - 2B adrenergic receptor, a G protein-coupled receptor. It is expressed in the central nervous system, blood vessels, and some other tissues. In the nervous system, activation of ADRA2B receptors inhibits neurotransmitter release and modulates neuronal activity, contributing to the regulation of attention, mood, and pain perception. In blood vessels, it causes vasoconstriction. ADRA2B receptor polymorphisms have been associated with individual differences in stress response, cognitive function, and susceptibility to certain diseases.
