Introduction
Recombinant CHO-K1 cells have been developed to stably overexpress human apelin receptor (AGTRL1) on their surface. These cells contain high levels of the G protein Gαi, which is crucial for coupling with the receptor in downstream signaling pathways.
Background
The AGTRL1 gene encodes the apelin receptor, a G protein-coupled receptor. It is expressed in various tissues, including the heart, blood vessels, lungs, and adipose tissue. Apelin, the ligand for the AGTRL1 receptor, has multiple physiological effects, such as promoting vasodilation, increasing cardiac contractility, regulating fluid balance, and influencing energy metabolism. The apelin - AGTRL1 system plays important roles in cardiovascular function, fluid homeostasis, and metabolic regulation. Dysfunction of this system may be involved in cardiovascular diseases, obesity, and diabetes.
