Introduction
Recombinant CHO-K1 cells have been created to stably overexpress human bombesin receptor 2 (BB2) on their surface. These cells contain high levels of the G protein Gαq, which is crucial for coupling with the receptor in downstream signaling pathways.
Background
The BB2 gene, also known as GRPR (gastrin - releasing peptide receptor), encodes a G protein - coupled receptor. It is widely expressed in the central nervous system, gastrointestinal tract, and other tissues. Activation of GRPR by gastrin - releasing peptide (GRP) regulates numerous functions, including neuronal excitation, smooth muscle contraction, and immune responses. In the brain, it is involved in processes such as pain perception, anxiety, and learning and memory.
