CHO-K1-BB3-Stable Cell Line
| Catalog Number |
CBCL-0075 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
Recombinant CHO-K1 cells have been developed to stably overexpress human bombesin receptor subtype 3 (BB3) on their surface. These cells contain high levels of the G protein Gαq, which is essential for coupling with the receptor in downstream signaling pathways.
Application
Drug screening and biological assays
Background
The BB3 gene, or BRS3 (bombesin receptor subtype 3), encodes a G protein - coupled receptor. It has a more restricted tissue distribution compared to BB1 and BB2. BRS3 is involved in the regulation of various physiological functions, including thermogenesis, energy balance, and cardiovascular function. Its activation can affect heart rate, blood pressure, and body temperature regulation.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-BB3-Stable Cell Line |
| Protein Family |
Bombesin receptor |
| Gene |
BB3/BRS3 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
