CHO-K1-CRF1/Gα15-Stable Cell Line
| Catalog Number |
CBCL-0093 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
A CHO-K1 cell line has been engineered to stably overexpress human corticotropin releasing hormone receptor 1 (CRF1) on its surface. This cell line contains high levels of the G protein Gαs, which is essential for coupling with the receptor in downstream signaling pathways.
Application
Drug screening and biological assays
Background
Corticotropin Releasing Hormone Receptor 1 (CRHR1) is a G protein-coupled receptor that binds corticotropin-releasing hormone (CRH) and related peptides, playing a crucial role in the stress response and regulation of the hypothalamic-pituitary-adrenal axis.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-CRF1/Gα15-Stable Cell Line |
| Protein Family |
Corticotropin releasing factor receptor |
| Gene |
CRF1/CRHR1 |
| NCBI Gene ID |
1394 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin, 100 μg/ml Hygromycin B |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
