CHO-K1-CT/Gα15-Stable Cell Line
| Catalog Number |
CBCL-0079 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
A CHO-K1 cell line has been created to stably overexpress human calcitonin receptor (CT) on its surface. This cell line contains high levels of both G protein Gαq and Gαs, which are essential for coupling with the receptor in downstream signaling pathways.
Application
Drug screening and biological assays
Background
The CALCR gene encodes the calcitonin receptor, which is a G protein - coupled receptor. Calcitonin, a hormone produced by the thyroid gland, binds to the CALCR receptor. Activation of the receptor leads to a decrease in blood calcium levels by inhibiting bone resorption and increasing calcium excretion by the kidneys. It also has effects on the gastrointestinal tract, such as reducing gastric acid secretion.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-CT/Gα15-Stable Cell Line |
| Protein Family |
Calcitonin receptor |
| Gene |
CALCR/CT |
| NCBI Gene ID |
799 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
