CHO-K1-CXCR1/Gα15-Stable Cell Line
| Catalog Number |
CBCL-0085 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
A CHO-K1 cell line has been developed to stably overexpress human C-X-C motif chemokine receptor 1 (CXCR1) on its surface. This cell line contains high levels of the G protein Gαi, which is crucial for coupling with the receptor in downstream signaling pathways.
Application
Drug screening and biological assays
Background
The CXCR1 gene, also known as IL8RA, encodes a chemokine receptor that is a G protein - coupled receptor. It is mainly expressed on neutrophils, as well as on some other immune cells. CXCR1 binds to interleukin - 8 (IL - 8) and other related chemokines. Activation of CXCR1 by these chemokines triggers neutrophil migration to sites of infection or inflammation, phagocytosis, and the release of reactive oxygen species, playing a critical role in the innate immune response against bacterial infections.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-CXCR1/Gα15-Stable Cell Line |
| Protein Family |
Chemokine receptor |
| Gene |
CXCR1/IL8RA |
| NCBI Gene ID |
3577 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 3 µg/ml puromycin, 100 µg/ml Hygromycin B |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
