CHO-K1-CXCR5/Gα15-Stable Cell Line
| Catalog Number |
CBCL-0088 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
A CHO-K1 cell line has been created to stably overexpress human C-X-C motif chemokine receptor 5 (CXCR5) on its surface. This cell line contains high levels of the G protein Gαi, which is essential for coupling with the receptor in downstream signaling pathways.
Application
Drug screening and biological assays
Background
The CXCR5 gene, also known as CD185, encodes a chemokine receptor that is mainly expressed on B lymphocytes and some follicular helper T cells. It binds to the chemokine CXCL13. Activation of CXCR5 by CXCL13 regulates B cell migration to lymphoid follicles, where B cells undergo maturation, antibody class - switching, and affinity maturation. CXCR5 plays a crucial role in the formation of germinal centers and the humoral immune response.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-CXCR5/Gα15-Stable Cell Line |
| Protein Family |
Chemokine receptor |
| Gene |
CXCR5/CD185 |
| NCBI Gene ID |
643 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 3 μg/ml puromycin, 100 μg/ml Hygromycin B |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
