CHO-K1-FFA1/Gα15-Stable Cell Line
| Catalog Number |
CBCL-0102 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
A CHO-K1 cell line has been created to stably overexpress human free fatty acid receptor 1 (FFA1) on its surface. This cell line contains high levels of the G protein Gαq, which is essential for coupling with the receptor in downstream signaling pathways.
Application
Drug screening and biological assays
Background
G protein-coupled receptor 40 (GPR40), also known as free fatty acid receptor 1 (FFA1), is a receptor for medium- and long-chain free fatty acids. It is expressed in pancreatic beta cells and plays a key role in regulating insulin secretion in response to increased plasma fatty acid levels.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-FFA1/Gα15-Stable Cell Line |
| Protein Family |
Free fatty acid receptor |
| Gene |
GPR40/FFA1 |
| NCBI Gene ID |
2864 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
