CHO-K1-Gα15/AMY2-Stable Cell Line
| Catalog Number |
CBCL-0077 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
A CHO-K1 cell line has been genetically engineered to stably overexpress human receptor activity modifying protein 1 (RAMP1) and calcitonin receptor (CTR) on its surface. Amylin receptors (AMYR), which are unique protein heterodimers consisting of a CTR paired with one of three receptor activity-modifying proteins (RAMP1, RAMP2, RAMP3), form three distinct subtypes (AMY1, AMY2, AMY3).
Application
Drug screening and biological assays
Background
The AMY2 gene encodes pancreatic amylase, which is secreted by the pancreas into the small intestine. It continues the digestion of starches that were initiated by salivary amylase. Pancreatic amylase breaks down starch into maltose and other small oligosaccharides, which are further digested by other enzymes in the small intestine.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-Gα15/AMY2-Stable Cell Line |
| Protein Family |
Calcitonin receptor |
| Gene |
AMY2 |
| NCBI Gene ID |
279 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B, 200 μg/ml Zeocin |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
