CHO-K1-Gα15/AMY3-Stable Cell Line

Catalog Number	CBCL-0078
Price	Online Inquiry
Unit Quantity	1 x 10^6 cells / 1.0 ml

Introduction

A CHO-K1 cell line has been developed to stably overexpress human receptor activity modifying protein 3 (RAMP3) and calcitonin receptor (CTR) on its surface. Similar to other amylin receptor configurations, these receptors are composed of a CTR combined with one of three receptor activity-modifying proteins (RAMP1, RAMP2, RAMP3), resulting in three subtypes (AMY1, AMY2, AMY3).

Application

Drug screening and biological assays

Background

Product Information

Category	GPCR Cell Lines
Cell Line Name	CHO-K1-Gα15/AMY3-Stable Cell Line
Protein Family	Calcitonin receptor
Gene	AMY3
Species	Hamster
Host Cell Line	CHOK1
Cell Type	Chinese hamster ovary cell line
Morphology	Epithelial
Growth Properties	Adherent
Recommended Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin, 400 μg/ml Geneticin, 100 μg/ml Hygromycin B
Freeze Medium	45% culture medium + 45% FBS + 10% DMSO
Storage Method	Frozen in liquid nitrogen.
Stability	Validated for at least 10 passages
Mycoplasma	Negative
Sterility Testing	Negative
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.