CHO-K1-GPBAR1-Stable Cell Line

Introduction

Recombinant CHO-K1 cells have been engineered to stably overexpress human G protein-coupled bile acid receptor 1 (GPBAR1) on their surface. These cells contain high levels of the G protein Gαs, which is essential for coupling with the receptor in downstream signaling pathways.

Application

Drug screening and biological assays

Background

The GPBAR1 gene encodes the G protein - coupled bile acid receptor 1, also known as TGR5. It is expressed in various tissues, including the gallbladder, intestine, liver, and immune cells. Activation of GPBAR1 by bile acids or synthetic agonists has multiple effects, such as stimulating the secretion of glucagon - like peptide - 1 (GLP - 1) from intestinal L cells, which promotes insulin secretion and regulates blood glucose levels. It also has anti - inflammatory effects in immune cells and plays a role in regulating energy expenditure and bile acid homeostasis. GPBAR1 is a potential target for the treatment of metabolic disorders such as diabetes and obesity.

Product Information