CHO-K1-IP1/Gα15-Stable Cell Line
| Catalog Number |
CBCL-0156 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
Recombinant CHO-K1 cells have been created to stably overexpress human prostaglandin I2 receptor (PTGIR) on their surface. These cells contain high levels of the G protein Gαs, which is essential for coupling with the receptor in downstream signaling pathways.
Application
Drug screening and biological assays
Background
PTGIR, or the prostaglandin I2 receptor, is a receptor for prostaglandin I2 (prostacyclin, PGI2). PGI2 is a potent vasodilator and inhibitor of platelet aggregation. Activation of PTGIR by PGI2 leads to increased cAMP production, which relaxes vascular smooth muscle and inhibits platelet activation. PTGIR is a target for drugs used to treat pulmonary arterial hypertension and other cardiovascular diseases.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-IP1/Gα15-Stable Cell Line |
| Protein Family |
Prostanoid receptor |
| Gene |
IP1/PTGIR |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin, 100 μg/ml Hygromycin B |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
