CHO-K1-KiSS1-Stable Cell Line
| Catalog Number |
CBCL-0115 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
Recombinant CHO-K1 cells have been created to stably overexpress the human KiSS1 receptor (KiSS1R) on their surface, and they contain high levels of the G protein Gαq, which is involved in coupling with the receptor to facilitate downstream signaling pathways.
Application
Drug screening and biological assays
Background
Kisspeptin receptor (GPR54) is a G protein-coupled receptor that binds kisspeptin, a peptide encoded by the KiSS1 gene. It plays a crucial role in the regulation of the reproductive axis by controlling gonadotropin-releasing hormone (GnRH) secretion.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-KiSS1-Stable Cell Line |
| Protein Family |
Kisspeptin receptor |
| Gene |
KiSS1/GPR54 |
| NCBI Gene ID |
84634 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
