Introduction
Recombinant CHO-K1 cells have been developed to stably overexpress human cholinergic receptor muscarinic 3 (M3) on their surface. These cells contain high levels of the G protein Gαq, which is essential for coupling with the receptor in downstream signaling pathways.
Background
The CHRM3 gene encodes the muscarinic acetylcholine receptor M3, which is a G protein-coupled receptor. It is widely distributed in various tissues, including smooth muscle, glands, and the central nervous system. Activation of M3 receptors leads to a variety of physiological responses, such as smooth muscle contraction (e.g., in the bronchi and gastrointestinal tract), glandular secretion (e.g., salivary and gastric glands), and modulation of neuronal activity. It plays important roles in regulating many bodily functions, including respiration, digestion, and autonomic nervous system activity.
