Introduction
Recombinant CHO-K1 cells have been engineered to stably overexpress human cholinergic receptor muscarinic 4 (M4) on their surface. These cells contain high levels of the G protein Gαi, which is crucial for coupling with the receptor in downstream signaling pathways.
Background
The CHRM4 gene encodes the muscarinic acetylcholine receptor M4, a G protein-coupled receptor. It is expressed in the central nervous system, particularly in areas involved in cognitive function, motor control, and reward pathways. M4 receptors are involved in modulating neurotransmitter release, such as dopamine and acetylcholine. They play a role in regulating learning, memory, attention, and motor behavior. Dysfunction of M4 receptors has been implicated in various neurological and psychiatric disorders, including Parkinson's disease and schizophrenia.
