CHO-K1-M5-Stable Cell Line
| Catalog Number |
CBCL-0057 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
Recombinant CHO-K1 cells have been created to stably overexpress human cholinergic receptor muscarinic 5 (M5) on their surface. These cells contain high levels of the G protein Gαq, which is essential for coupling with the receptor in downstream signaling pathways.
Application
Drug screening and biological assays
Background
The CHRM5 gene encodes the muscarinic acetylcholine receptor M5, a G protein-coupled receptor. It is mainly expressed in the central nervous system, especially in the substantia nigra, ventral tegmental area, and hippocampus. M5 receptors are involved in regulating dopamine release and neuronal excitability. They play roles in motor control, reward - related behaviors, and cognitive functions. Alterations in M5 receptor function may contribute to the pathophysiology of neurological disorders such as Parkinson's disease and addiction.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-M5-Stable Cell Line |
| Protein Family |
Acetylcholine receptor (muscarinic) |
| Gene |
M5/CHRM5 |
| NCBI Gene ID |
1133 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
