CHO-K1-PAC1/Gα15-Stable Cell Line
| Catalog Number |
CBCL-0175 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
Recombinant CHO-K1 cells have been developed to stably overexpress the human pituitary adenylate cyclase-activating polypeptide type I receptor (PAC1) on their surface, and they contain high levels of the G proteins Gαq and Gαs, which are involved in coupling with the receptor to facilitate downstream signaling pathways.
Application
Drug screening and biological assays
Background
Pituitary adenylate cyclase-activating polypeptide type 1 receptor (PAC1R) is a G protein-coupled receptor that binds pituitary adenylate cyclase-activating polypeptide (PACAP), a neuropeptide involved in neurotransmission, neuroendocrine regulation, and neuroprotection.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-PAC1/Gα15-Stable Cell Line |
| Protein Family |
VIP and PACAP receptor |
| Gene |
PAC1R |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin, 100 μg/ml Hygromycin B |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
