CHO-K1-PTH1/Gα15-Stable Cell Line
| Catalog Number |
CBCL-0149 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
Recombinant CHO-K1 cells have been engineered to stably overexpress human parathyroid hormone 1 receptor (PTH1) on their surface. These cells contain high levels of both G protein Gαs and Gαq, which are crucial for coupling with the receptor in downstream signaling pathways.
Application
Drug screening and biological assays
Background
PTHR1, also known as the parathyroid hormone 1 receptor, is a G-protein coupled receptor that binds both parathyroid hormone (PTH) and parathyroid hormone-related protein (PTHrP). It plays a pivotal role in calcium homeostasis by regulating bone resorption, renal calcium reabsorption, and intestinal calcium absorption. Dysregulation of PTHR1 signaling can lead to disorders such as hyperparathyroidism and hypoparathyroidism, which affect calcium levels in the body.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-PTH1/Gα15-Stable Cell Line |
| Protein Family |
Parathyroid hormone receptor |
| Gene |
PTH1/PTHR1 |
| NCBI Gene ID |
5741 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin, 100 μg/ml Hygromycin B |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
