CHO-K1-SST5/Gα15-Stable Cell Line
| Catalog Number |
CBCL-0166 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
Recombinant CHO-K1 cells have been developed to stably overexpress the human somatostatin receptor 5 (SST5) on their surface, and they contain high levels of the G protein Gαi, which is involved in coupling with the receptor to facilitate downstream signaling pathways.
Application
Drug screening and biological assays
Background
SSTR5, or the somatostatin receptor 5, is a G-protein coupled receptor that binds somatostatin with high affinity. It is primarily expressed in the pituitary gland and pancreas, where it regulates growth hormone and insulin secretion, respectively. SSTR5 activation leads to decreased intracellular cAMP levels and inhibition of cell proliferation. It is a target for somatostatin analogs used to treat acromegaly and other hormone-secreting tumors.
Product Information
| Category |
GPCR Cell Lines |
| Cell Line Name |
CHO-K1-SST5/Gα15-Stable Cell Line |
| Protein Family |
Somatostatin receptor |
| Gene |
SST5/SSTR5 |
| NCBI Gene ID |
6755 |
| Species |
Hamster |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B |
| Freeze Medium |
45% culture medium + 45% FBS + 10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
