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CLDN18.2 Stable NCI-N87 Cell Line

Catalog Number CBCL-542
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

NCI-N87 cell line stable expressing exogenous human CLND18 gene isoform 2

Application

Drug screening and biological assays

Background

CLDN18.2 is the isoform 2 of the claudin 18 gene, which belong to the lager claudin family and play the important role in cell tight junction in epithelial cells. The identification as a tumor target of CLDN18.2 has led to the repaid progress of antibody treatment of gastrointestinal adenocarcinoma and pancreatic tumors.

Product Information

Species Human
Host Cell Line NCI-N87
Cell Type Gastric tubular adenocarcinoma cell line
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium RPMI1640 + 10% FBS + 1 μg/ml Puromycin
Selection Marker Puromycin
Morphology Monolayer
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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