Catalog Number | CBCL-3 |
Price | Online Inquiry |
Unit Quantity | 1x106 cells / 1.0 ml |
Stable Ba/F3 clone expressing exogenous CLIP1-LTK fusion gene.
Drug screening and biological assays
LTK is a member of the anaplastic lymphoma kinase (ALK)/LTK subfamily within the insulin receptor superfamily of RTKs. Although LTK can activate the RAS/MAPK and PI3K/AKT pathways and is expressed in several organs, including the brain, bone marrow, lymphoid tissues, and placenta. In malignancies, LTK has been found to be overexpressed in leukemia, and high expression of LTK in early-stage NSCLC has been associated with a greater risk of metastasis. Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.
Category | Kinase Cell Lines |
Cell Line Name | CLIP1-LTK Stable Ba/F3 Cell Line |
Pharmaceutical Target | Kinases |
Species | Mouse |
Host Cell Line | Ba/F3 |
Cell Type | Pro-B cells |
Morphology | Mostly round |
Growth Properties | Suspension |
Selection Marker | Puromycin |
Seeding Density | Thaw entire contents into an appropriate T25 flask. |
Subculture Ratio | Recommended a ratio of 1:10. |
Doubling Time | Approximately 20 hours |
Recommended Medium | RPMI 1640 + 10% FBS |
Storage Method | Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
Stability | Stable in culture over 10 passages |
QC | WB |
Shipping | Shipping in dry ice |
Biosafety Level | BSL-1 |
Caution | For Research Use Only |