CSF1R Stable Ba/F3 Cell Line
| Catalog Number |
CBCL-149 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
Stable Ba/F3 clone expressing exogenous human CSF1R gene.
Application
Drug screening and biological assays
Background
Colony stimulating factor 1 receptor (CSF1R) is a class III receptor tyrosine kinase that is expressed on the cell surface of microglia and macrophages.Mutations in CSF1R are also associated with CML and type M4 AML. The identification of CSF1R as drug target has led to repaid development of the inhibitor of CSF1R in the treatment of cancer or inflammatory diseases.
Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.
Product Information
| Species |
Mouse |
| Host Cell Line |
Ba/F3 cell line |
| Cell Type |
Pro-B cells |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Suspension |
| Pharmaceutical Target |
Kinases |
| Recommended Medium |
RPMI 1640 + 10% FBS + 10000U/ml CSF1 |
| Selection Marker |
Puromycin |
| Morphology |
Mostly round |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:10. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 20 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
