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Cyno CD122 Stable 293T Cell Line

Catalog Number CBCL-499
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

HEK293T cell line stable expressing exogenous cyno CD122 gene

Application

Drug screening and biological assays

Background

D122, also named as Interleukin-2 receptor subunit beta (IL2RB), which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. This protein also forms one of the three subunits of the IL-15 receptor. Activation of the receptor increases proliferation of CD8+ effector T cells.

Product Information

Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium DMEM + 10% FBS + 0.5 μg/ml Puromycin
Selection Marker Puromycin
Morphology Monolayer
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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