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Cyno CD25 Stable 293T Cell Line

Catalog Number CBCL-384
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable 293T cell line expressing exogenous CD25 gene

Application

Drug screening and biological assays

Background

CD25 (a chain of the high-affinity IL-2 receptor) deficiency is an autosomal recessive disorder due to mutations in the IL2RA gene. T regulatory cells constitutively express CD25 and respond to IL-2 generated by T cells during an immune response for their immunoregulatory functions. CD25 deficiency results in a syndrome similar to IPEX with severe enteropathy, diabetes mellitus, autoimmune hemolytic anemia, eczema, and lymphoproliferation.

Product Information

Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium DMEM + 10% FBS + 1 μg/ml Puromycin
Selection Marker Puromycin and hygromycin B
Morphology Epithelial
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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