Cyno CD25 Stable 293T Cell Line
| Catalog Number |
CBCL-384 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
Stable 293T cell line expressing exogenous CD25 gene
Application
Drug screening and biological assays
Background
CD25 (a chain of the high-affinity IL-2 receptor) deficiency is an autosomal recessive disorder due to mutations in the IL2RA gene. T regulatory cells constitutively express CD25 and respond to IL-2 generated by T cells during an immune response for their immunoregulatory functions. CD25 deficiency results in a syndrome similar to IPEX with severe enteropathy, diabetes mellitus, autoimmune hemolytic anemia, eczema, and lymphoproliferation.
Product Information
| Species |
Human |
| Host Cell Line |
293T |
| Cell Type |
Human embryonic kidney cells |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
DMEM + 10% FBS + 1 μg/ml Puromycin |
| Selection Marker |
Puromycin and hygromycin B |
| Morphology |
Epithelial |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:5. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 30 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
